Laboratories

Laboratory of functional Genomics does not recruit students for the academic year 2018

Professor Sumio SUGANO 
+81-3-5449-5286
E-mail: ssugano{at}k.u-tokyo.ac.jp
Lab HP



Associate Professor Manabu WATANABE
+81-3-5449-5343
wata{at}k.u-tokyo.ac.jp

Introduction

 The aim of our lab is to develop and apply new genome analysis methods in order to understand disease processes especially that of cancer. Living organism can be seen as a system consists of tens of thousands of components called genes and the disease as the malfunction of the system. To understand the system, we first have to identify the genes. Then, the analysis of individual function of the genes and the relationship between them can be studied. We devised a method,so called“oligocapping”, to mark the 5’end of mRNA.


Full-leng th enriched cDNA library by Oligo -capping method


 Using the method, we made various full-length enriched cDNA libraries and isolated almost all the protein coding genes of human, 20-25% of them are still “function unknown”. We also fund there are numerous non-coding RNAs. Although the function of the most of them is still not understood, at least some of them have important role in normal and disease process as protein coding genes




Sub-cellular localization of function unknown genes



 We also combined our oligo-capping method with Next Generation Sequencers and devised a high-throughput method to map transcriptional start sites (TSS) in genome wide manner. The data obtained by this TSS-seq method allow us to see how individual (alternative) promoter fire during developments or during disease process. Many such data were collected and made available to the research community through our database DBTSS (http://dbtss.hgc.jp.).
We now applying TSS-seq as well as other methods such as RNA-seq,ChIP-seq,SNV or CNV analysis to the clinical samples from cancer patients. Using data obtained,we are identifying potential diagnostic or clinical target genes.

DBTSS :database for transcriptional start sites (http://dbtss.hgc.jp/)

 We now applying TSS-seq as well as other methods such as RNA-seq,ChIP-seq,SNV or CNV analysis to the clinical samples from cancer patients. Using data obtained,we are identifying potential diagnostic or clinical target genes.

Laboratories

The University of Tokyo
Graduate School of Frontier Sciences, The University of Tokyo

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