Laboratories

Laboratory of Biomolecules Recruiting students for the academic year 2018

Profesor Takuya UEDA
+81-4-7136-3641
E-mail: ueda{at}k.u-tokyo.ac.jp
Lab HP

Associate Professor Nono TOMITA
+81-4-7136-3644
E-mail: nono{at}k.u-tokyo.ac.jp

Introduction

【Key Words】translation system,ribosome, tRNA, yeast, mitochondria

 Protein synthesis is the most fundamental process in life. We address elucidation of a basic principle of translation systems derived various living organisms, such as bacteria, eukaryote and mitochondria. Based on cell-free translation system, we are challenging creation of artificial cell, one goal of Synthetic Biology. Moreover, the development of drugs discovery system using the PURE ribosome display method is addressed.

(1) Biochemistry of translation system

 Using the reconstituted translation systems (PURE systems) originated of E. coli, yeast and mitochondria, we are promoting biochemical studies to elucidate more precise mechanism of translation process. Recently, yeast PURE system was successfully reconstituted and is now clarifying various regulation mechanism in translational process, such as mRNA-quality control. Studies on animal mitochondrial translation system are also in progress,which will provide us useful clues for the therapy of mitochondrial disease

PURE system

(2)Artificial cell based on the PURE system:

 All the proteins encoded on E. coli genome have been successfully expressed using the PURE system. We are also developing the cell-free system capable of producing complicated complex in cells, such as ATPase on membrane and ribosomal subunits. Through these bottom-up approaches, we would like pave the way to experimental constitution of cell.

Artificial cell


(3)Development of medical probesusing cell-free system:

 We are exploring protein-binders including antibodies by the ribosome-display method based on the PURE system. The peptide-drugs targeting GPCR synthesized by the PURE system will be eveloped by the in vitro evolution system.

PURE ribosome display method

Referrences:

 Nat Biotechnol (2001)19, 751-755
 Mol Cell (2009)35, 502-510
 Angew Chem Int Ed Engl (2014)53, 7535-7538
 Biochem J (2012)442, 631-638
 PNAS (2009)106, 4201-4206
 PNAS (2012)109, 8937-8942
 BBRC (2007)352, 270-276
 BBRC (2012)428, 395-400

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